In the saline group, some neurons and neuronal nuclei in addition to the SGCs (arrows) displayed immunoreactivity.
We found homogenous DREAM immunoreactivity in the cytoplasm of some neurons and in the neuronal nuclei of fresh TG (Additional file 3: Figure S3a).
b At 2 days post-CFA injection, positive neuronal nuclei (thin arrow), a few neurons (thin arrowhead) and many SGCs (thick arrow) were present.
a In fresh trigeminal ganglia homogenous DREAM immunoreactivity was found in the cytoplasm of some neurons (arrow head) and in the neuronal nuclei (thin arrow).
We labeled neuronal nuclei and the surrounding cytosol by the neuron-specific nuclear protein "Neuronal Nuclei" (NeuN; Mullen et al. 1992) in serial sections of the barrel field cut in the tangential plane.
Landry, C.F. et al. Myelin basic protein gene expression in neurons: developmental and regional changes in protein targeting within neuronal nuclei, cell bodies, and processes.
Neurons were stained with a mouse polyclonal antibody directed against Neuronal Nuclei, NeuN (1∶10,000, Chemicon, Billerica, Massachusetts).
To determine the proportion of neurons produced, colabeling was assessed between BrdU and the mature neuronal marker neuronal nuclei (NeuN).
BrdU, bromodeoxyuridine; NeuN, neuronal nuclei; TAM, Tamoxifen.
Immunofluorescence experiments to visualize neuronal nuclei with the Hoechst dye were performed as previously described36.
BrdU, bromodeoxyuridine; CORT, corticosterone; DCX, doublecortin; NeuN, neuronal nuclei; TAM, Tamoxifen.
