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This neuronal length measurement method could supplement already existing neuropathological criteria for postmortem Alzheimer disease diagnostics.
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Decrease on neuronal process length represents a prominent feature of the degenerative phenotype associated with PD-associated LRRK2 mutations.
Reductions of hippocampal neuronal dendritic length and complexity were found in association with aging and stress [23], [24].
Inhibition of macroautophagy in differentiated SH-SY5Y cells reversed the detrimental effects of mutant G2019S LRRK2 on neuronal process length [54].
The upregulation of soluble Ndel1 in injured nerves again led to enhanced formation of the neuronal Ndel1/full length Vimentin complex, as determined by co-immunoprecipitations with Ndel1 antibodies (Fig. 3B).
Auditory hair cell survival, spiral ganglion neuron number, length of neurites, and neuronal survival were analyzed in vitro.
Neuronal survival, neurite length, and branching were examined as an initial comparison.
To further confirm that DR6 expression level contributes to neuronal death, full-length DR6 (DR6 FL) was introduced into neocortical neurons by lentivirus infection.
Glutamate excitotoxicity is characterized morphologically by a decrease in the number of neurons and a reduction in the length of neuronal processes.
Since these terminal branches from non-clear sources are excluded from the analyses, tracings from these experiments tend to underestimate the real complexity of the neurons, yielding lower values for total dendritic length and neuronal complexity.
In line with these findings, we observed the secretion of GDNF, BDNF, NT-3, and b-NFG by undifferentiated hDPSCs and their beneficial effects on neuronal survival and neurite length of cultured dissociated DRG neurons.
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