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For neuronal differentiation, the mES cells were differentiated in monolayer.
The standard substrate for neuronal differentiation, PLO, supported moderate neuronal differentiation compared to silk-CNT scaffolds.
Neuronal differentiation is an early and fundamental event in neurodevelopment.
Cells on silk scaffolds exhibited limited neuronal differentiation.
(A) Schematic illustration of neuronal differentiation from NSCs.
GAP43 was used as a neuronal differentiation marker.
Expectedly, immobilized IFN-γ increased neuronal differentiation on both materials.
Compared to –COOH surfaces, –NH2 seemed to promote neuronal differentiation.
We observed that knockdown of TET1, TET2 or TET3 promoted neuronal differentiation of Neuro2a cells, and their overexpression inhibited VPA (valproic acid -induced neuronacid -inducedationeuronalsting all three TET proteins negatively regulate neuronal differentiation of Neuro2a cellsuggesting
Our previous studies have demonstrated that srGAP3 can negatively regulate neuronal differentiation of Neuro2a cells.
(F) A proposed working model for TET1 functions in regulating neuronal differentiation.
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