But as it briskly shows their progress, broken up by dippy animations of neuron storms, it feels like a demonstration of a point.
The reconstructed neuron image, 3D STORM images of axon and dendrite from indicated regions (arrows), and autocorrelation analyses are shown.
The reconstructed neuron image, 3D STORM images of indicated regions (arrows), and corresponding autocorrelation analyses of DIV 4 (A ) and DIV 8 (B ) neurons are shown.
Because the closely related neuropeptide VIP suppresses the sIAHP by activating the cAMP/PKA pathway in CA1 pyramidal neurons (Haug and Storm, 2000) and PACAP has been reported to do the same in the neocortex (Hu et al., 2011), we initially expected PACAP to exert its inhibitory action on the sIAHP predominantly through the same pathway.
In addition, several of these 14 neurons can predict big magnetic storm (Dst ⩽ −180 nT).
Zhuang and co-workers recently addressed this question using a super-resolution microscopy technique called stochastic optical reconstruction microscopy (STORM) to examine neurons in cell culture and in brain slices (Xu et al., 2013).
A representative STORM image of a treated neuron at DIV 6, the enlarged images of the boxed regions, and the autocorrelation analyses (n = 10 neurons, four independent experiments) are shown.
To address this question, we performed STORM imaging on disassociated hippocampal neurons from ankyrin B knockout mice (Scotland et al., 1998) at DIV 10.
We have observed this periodic membrane skeleton with STORM imaging of fixed cultured neurons and fixed brain tissue slices (Xu et al., 2013) using actin-binding phalloidin and immunolabeling of endogenously expressed proteins, as well as in live cultured neurons using fluorescent fusion proteins.
To test this hypothesis, we increased the expression level of βII spectrin in all neurites by transiently transfecting neurons with a HA-tagged βII spectrin construct and performed STORM imaging on βII spectrin in transfected neurons at DIV 11.
