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Simulations were done using NEURON software [33], [34] and the SG neuron model from [23] (http://senselab.med.yale.edu/ModelDb, 62285).
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Estimation of the number of cells immunopositive for leptin was performed using neurolucida neuron Tracing Software (MBF Bioscience).
In 19 from a total of 114 recorded cells, the morphology of neurons was reconstructed using a Neurolucida (MicroBrightfield, V7) and in three neurons ImageJ software (V1.47).
To quantify PKA activity in neurons, Metamorph software (Molecular Devices) was used to create a binary mask of the cells from the mVenus images.
We plan to eventually release the software as a plugin of the V3D-Neuron package at http://penglab.janelia.org/proj/v3d.org/proj/v3d
The dendritic and axonal length of all neurons was measured in a 50 µm × 50 µm Cartesian grid, yielding a raw density map of the dendritic arborization and axonal collateralization by using the open-source software NEURON (http://www.neuron.yale.edu).edu
Sholl analysis was performed on TH+/MAP+ neurons using NIH ImageJ software with a Sholl analysis plug-in (v1.0) developed by the laboratory of Anirvan Ghosh (http://www.biology.ucsd.edu/labs/ghosh/software/index.html) [60] to quantify neuritic complexity.
Another blinded investigator measured these marked neurons using ImageJ software.
Images were processed and superimposed to identify double-labeled neurons using Photoshop software (Version CS5.1).
We prepared hippocampal slices from 3-month-old animals, and traced individual CA1 neurons using Neurolucida software.
And Audi is also rumoured to be working with Axeon.The neural networks in question are either dedicated silicon chips (such as those made by Axeon) with neurons modelled in hardware, or software models of neurons implemented on standard microprocessor chips.
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