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The DRRC units are developed based on the methodology of neuron configuration, i.e., one neuron or multiple neurons.
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While this can be performed analytically in simple electrode-neuron configurations, numerical models are required for realistic geometries.
The binding of novel constellations, by contrast, is assumed to be achieved by the formation of functionally coherent assemblies of neurons whose configuration results from a self-organizing process and can change in a context-dependent way.
As the expression is always in CA1 hippocampal pyramidal neurons and the stimulation is applied on Schaffer collateral fibres from CA3 neurons, this experimental configuration ensures that the potential effects of the expressed recombinant proteins are postsynaptic.
Many central pattern generators (CPGs) are built on a basic circuit of reciprocally inhibitory neurons, the minimal configuration that can produce distinct rhythmic patterns for controlling antagonistic muscles.
After obtaining the whole cell configuration, neurons were held routinely at -70 mV for EPSCs or at 0 mV for IPSCs, respectively.
Conventional whole-cell patch clamp recordings were performed in the primary cultured hippocampal neurons under standard configuration.
This study only included neurons whose spike configuration remained constant and could be clearly discriminated from activity in the background throughout the experiment, indicating that the activity from one neuron only and from the same neuron was measured.
This study only included neurons whose spike configuration remained constant and could clearly be discriminated from activity in the background throughout the experiment, indicating that the activity from one neuron only and from that same neuron was measured.
For each neuron and each stimulation configuration, the extracellular potential computed in the finite element model (without offset correction) was interpolated at the center of each compartment and assigned with the extracellular mechanism.
We also examined spontaneous fast current transients (CTs) recorded from these pyramidal neurons under cell-attached configuration.
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