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Fish were immersed in 4.5% (w/v) NaCl (1450 mOsm/kg aerated overnight before use) for 2 min and immediately net transferred to vaccine solution (LPS DTAF (0.2% (w/v)), A. salmonicida bacterin-FITC (2.4×107 bacteria/ml) or BSA-FITC (2% (w/v)) for 10 min (HI, hyperosmotic immersion, fish).
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What really counts is net transfers.
Their net transfer spend since 2010-11 is close to zero.
Last year the east received net transfers of euro75 billion.
"But," Manfred added, and it was a major but, "the net transfer is half the issue.
A commission paper circulated at the October 13th meeting offered no figures for net transfers.
There was a net transfer spend of £58m, taking total losses to almost £100m.
But the net transfer to wealthy countries amounted to $450 billion, three-fourths of which was absorbed by the United States.
In the seven years that followed, the country received some €30 billion (at 2004 prices) in net transfers.
In the years 1998-2005 the club's net transfer spend was £129m, 12 per cent of revenue.
What we see is that federal government spending results in large and persistent net transfers from some states to others.
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CEO of Professional Science Editing for Scientists @ prosciediting.com