The amounts of GST, GST-V12 Cdc42, GST-V12 Rand and GST-V12 Ras used in each assay were similar, as shown.
The generation of GST, GST-p300-CH3, GST-Bmi1 and GST-SYT-SSX2 lysates was performed as previously described [28].
The production of GST and different GST-SH2 fusion proteins [GST-AblSH2, GST-CrkLSH2, GST-FynSH2, GST-GapSH2 (N-terminal), GST-GrapSH2, GST-LckSH2, GST-Mona(Gads)SH2, GST-NckSH2, GST-PI3KSH2 (C-terminal), GST-PLCgammaSH2 (N-terminal), GST-ShcSH2, GST-SrcSH2, GST-Shp2SH2 (C-terminal), GST-TensinSH2 and GST-Vav1SH2] was described previously [ 40- 43].
In all cases 100 ng of GST-ATXN1 30Q) GST-ATXN1 30Q82Q) were used for transfection.
(C) Coomassie blue staining of GST and GST-EB1 proteins purified from E. coli.
Commercially available GST-tag assays are designed for quantitative measurement of GST activity under native conditions.
The broad substrate acceptance of GST A1-1 contrasts with the high selectivity of GST A4-4 and GIMFhelix for alkenal substrates.
Immunoblotting analysis using the anti-GST and anti-MBP antibodies indicated a proper load of GST, GST-fused different portions of APIP12, and MBP-AvrPiz-t in the input (Fig. 2b).
However, the activity of GST was found to significantly increase in NATE treated group, probably due to up regulation of GST by the phytoconstituents of NATE.
An up-regulation of GST was also observed in Collembola treated with AgNPs and AgNO3.
The purities of GST and GST-dysbindin-1 are shown in Fig. 1A.
