Sentence examples for net buffer from inspiring English sources

48 h after transfection, cells were rinsed with phosphate-buffered saline (PBS), lysed in 200 μl NET buffer (50 mM Tris pH 7.4, 150 mM NaCl, 15 mM EDTA and 1% NP-40) and centrifugated for 10' at 13,000 g.

While the TFC is performed, every frame captured on the specific channel is stored in Net Buffer List (NBL), and then the user application refers the captured frame by reading the address of the NBL as in Figure 3(b).

Under PDOBR, the output buffer at every node of the ATM network is organized in the form of separate buffers for each output link, of size SN cells, plus a "floating" buffer of the same size, that may be appended, at runtime, to any one of the output links to augment its net buffer capacity.

The samples were washed four times in NET buffer.

For Western blot, cells were lysed with SDS buffer or NP40 in NET buffer.

PKDL tissue was collected in NET buffer [150 mM NaCl, 15 mM Tris-HCl (pH 8.30) and 1 mM EDTA].

In the other countries, more generous social safety nets buffer families from the health consequences of poverty, the report said.

The Sepharose was then collected, washed 4 times with NET-2 buffer, and then resuspended in 300 μl of NET-2 buffer.

Unbound extract (100 µl out of 400 µl) was kept for RNA extraction; beads were washed 8 times with 500 µl Net-2 buffer.

The protein-A sepharose was then washed 5 times with NET-N buffer and the boiled immunoprecipitates were subjected to SDS-PAGE analysis.

Transfected HEK293T cells were lysed with NET-N buffer (25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 0.2 mM EDTA, 0.1% NP40) and a cocktail of protease inhibitors (Roche, Mannheim, Germany) on ice for 20 minutes.