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Solutions were passed through nest substrate to simulate alterations in composition during percolation to egg depth.
Using data from ∼1400 nests of 23 species, we generated nest survival curves for groups of altricial species defined by nest substrate (ground, shrub, tree, or culvert).
Addition of dispersant increased tPAH prior to percolation in the High treatment (302 mg/L) relative to Low (13 mg/L), but percolation resulted in nearly equal concentrations in both treatments (High, 30; Low, 22 mg/L) due to physical trapping of dispersed oil by the nest substrate.
Remaining undeveloped eggs (opaque in appearance) were removed from the nest substrate and combined with the hatched larvae.
The bottom of the mesocosm was covered with cobble (∼5 10 cm diameter; ∼40 cm deep) and large rocks (20 30 cm diameter) to naturalise the breeding channels and provide the salmon with nest substrate.
Nest substrate had a large effect on nest success: despite their shorter nest exposure period, ground nests were least successful and culvert nests were most successful.
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Spruce seedlings grew better and had a more diverse spectra of EcM fungi in the ant nest substrates than in the forest floor substrate.
It is also plausible that the relationship between DSR of nests and nest age could be a function of predators cuing in on the nest due to olfaction from the female (Storaas 1988) because more odor will be emitted and bound to nest substrates (Conover 2007) the longer a female remains in one area (i.e., nest site).
The AG mutualism also makes it possible for the ants to colonize resource rich microhabitats independently of pre-existing nest substrates, an advantage that may have led to the dominance of AG ants in lowland Amazonia [5], [16].
Certain ecological traits of species were consistently associated with the responses to patch area across seasons (migratory strategy, nesting substrate and foraging trait), while body weight was only associated in the winter.
Interestingly, random site effects (e.g., site size, nesting substrate, type of predator fencing, monitoring frequency) explained negligible amounts of the variance in clutch size and fledgling productivity.
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