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Optic nerves were dissected, fixed in 2% osmium tetroxide, and embedded in epon resin.
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The left sciatic nerve was dissected, fixed in 4% paraformaldehyde solution for 48 h, conventionally dehydrated, cleared, and embedded in paraffin at a 4 μm thickness.
Mice liver tissues were dissected, fixed in 10% buffered formalin, and embedded in paraffin.
After 12 weeks, animals were sacrificed and retinas were dissected, fixed and sectioned.
The tumors were dissected, fixed and then sectioned for immunohistochemistry.
Tumors were dissected, fixed in formalin and embedded in paraffin.
Subcutaneous WAT were dissected, fixed, embedded in paraffin, and sectioned.
Briefly, brains of E18.5 embryos were dissected, fixed in Bouin's solution and embedded in paraffin.
Imaginal wing discs were dissected, fixed and stained as described in [56].
Wandering third instar larvae were dissected, fixed and stained as previously described [13].
For histomorphometric analysis, femurs were dissected, fixed, and embedded in methyl methacrylate.
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