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The corneal confocal microscopy showed the presence of activated keratocytes, while the nerve pattern was normal.
To circumvent these caveats and examine signalling modalities of HGF/Met in a system where they exert direct control of axonal growth and guidance, we chose to investigate the motor nerve pattern in the dorsal hindlimb.
They allow unambiguous uncoupling of Met requirements for axonal growth from muscle development, and they also allow distinguishing of a nerve pattern defect from the consequences of an altered MN specification process (unlike for brachial Pea3 MN pools).
However, in the presence of equivalent amounts of muscle mass, such branches are unequivocally detected in met 2P/2P but not in met 2S/2S embryos, where the nerve pattern is indistinguishable from that seen in met d/d mutants.
While examining the nerve pattern, we found that the peroneal nerve of met 2P/2P embryos had a shape similar to that of control embryos, with an average α of approximately 135°, and its side branches in contact with EDL and TA were present in most cases (with an average number of eight side branches per nerve compared to nine per nerve in controls; Figure 3F).
Similar(55)
The pattern of the autonomic innervation of the trabecular muscle of the Lepisosteus respiratory bladder is basically similar to that of the tetrapod lung with overlapping of both muscle architecture and control nerve patterns.
The patterns generated by these thoughts, along with nerve patterns associated with facial movement, are then coupled with various commands within the BMI software in order to control the computer.
For successful regeneration to occur, proper nerve patterning is essential, so we examined whether Arch expression correlated with the position of neurons.
Furthermore, they indicate that distinct signalling cascades, such as those triggered by these two receptor variants, are not equivalent in their capacity to execute nerve patterning by Met.
We also examined the effect of Arch stimulation on cell proliferation and nerve patterning, two processes that are required for regeneration.
Light-dependent Arch activation more than doubled the number of mitotic cells in the tail regeneration bud region (n = 22, t = 5.273, P<0.001) (Fig. 4E) and promoted proper nerve patterning (compare Fig. 4F,G to Fig. 4H,I).
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