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Histological analysis of the ocular tissue of control embryos at stages E11-14 (Fig. 1A C) showed a well-developed bi-layered optic cup, in which the inner or neural layer (the future nerve layer of the retina) and the outer layer (the future pigment layer of the retina) could be clearly discerned (Fig. 1D F).
In most cases the glomeruli appeared closely apposed to the NCAM+ nerve layer of the OB, but there were notable exceptions.
In some studies limited purification procedures have been carried out, for example the generation of cells from embryonic day 18 rat embryos by careful dissection of the olfactory nerve layer of the olfactory bulb (Doucette, 1993).
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Fucα(1−2 Gal sugars were expressed in both the glomerular and olfactory nerve layers (ONL) of the developing and adult OB.
A steak, for instance, consists of tens of thousands of muscle fibres, blood vessels, nerves, layers of fat and connective tissue, gristle and perhaps bone.
Furthermore, imaging of VNO axons and the AOB nerve layer illustrates strong enrichment of Dcx-DsRed axons in both structures (Fig. 2a).
Third, we cannot exclude the possibility that electrical stimulation applied to the optic nerve layer excited axon collaterals of sSC neurons, which did not express eNpHR.
Initial steps involved peeling away the olfactory nerve layer from the rest of the bulb and digesting the tissue in MEM-H containing 0.03% collagenase and 0.25% trypsin for 15 min at 37°C.
This corresponded to marked bilateral atrophy of the olfactory nerve layer and adjacent glomerular layer comprising the outer two tissue layers of the OB in SG-instilled mice.
Accordingly, Daam1 protein was detected in the lens of embryos at E12.5, especially in the cuboidal epithelium of the capsule, in the optic nerve and in both layers of retina – the nerve layer and the pigment layer (Fig. 2F).
The lesions on the right recurrent nerve, muscular layer of the esophagus, and the membranous wall of trachea were all resected with the tumor during the initial operation.
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