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The distribution of the nerve fiber diameter) revealed a comparable diameter distribution between the three groups especially for small diameter nerve fibers.
The two-dimensional dissector method (subarea 150 × 150 μm, subfields 50 × 50 μm) was used to determine the number of myelinated axons per cross-sectional area, nerve cross-sectional area, nerve fiber density, nerve fiber diameter, and nerve fiber area [ 39].
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Mean subbasal nerve fiber density near the corneal apex was 45.94 ± 5.20 mm/mm2 and mean subbasal and interconnecting nerve fiber diameters in the same region were 1.51 ± 0.74 μm and 0.69 ± 0.26 μm, respectively.
The total nerve area was traced from the overview micrographs and myelin rings were traced from at least 10% of the total nerve area; fiber diameters were calculated as the diameter of the circle having the same area as the outer contours of the "myelin rings".
The nerves of the Low-TENS group showed myelinated nerve fibers with diameters similar to those of the control nerves.
High-TENS nerves also presented lower fiber diameter (Kruskal-Wallis = 11.71, P < 0.01, Dunn P < 0.05)) and lower myelin area (Kruskal-Wallis = 7.96, P < 0.05, Dunn P < 0.05) and 3(4a)).
In randomly selected high power fields, the total number of nerve fibers, myelin sheath thickness and myelinated fiber diameter of the regenerated nerves were analyzed with Image Pro Plus 6.0.
We further found that the loss of the myelinated fibers length was mainly due to the marked loss of the myelinated nerve fibers with the diameter of 0.4 0.5 μm.
At 4, 8, and 16 weeks, significant differences in the thickness of myelin sheath of new myelinated nerve fibers and the diameter of axons were detectable between both groups (P < 0.05; Tables 4 and 5).
As expected, regenerated nerves showed smaller axon and fiber diameter and thinner myelin thickness.
To investigate the retinal vessel diameter and evaluate the relationship of the retinal nerve fiber layer (RNFL) thickness with retinal vessel diameter and intraocular pressure (IOP) in patients with normal-tension glaucoma (NTG).
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