Analysis of cranial nerve islet1 expression in isl1-GFP transgenic fish reveals that motor nerve III and IV were poorly detectable, and V and VII were reduced in size and disorganized in nestin morphants (Fig. 6B).
Thus, to identify miRNA regulators for genes in IRGCs, we analyzed the nerve miRNA expression measured before and after crush injury [ 6].
In the rat optic nerve, Bit1 expression was evident by E19; by P3, Bit1 and glial fibrillary acidic protein (GFAP) were strongly co-expressed, indicating that mature astrocytes express Bit1.
Interestingly, we found that several transcripts (Oct6, Egr2, Brn2, Sox10) encoding pro-myelinating proteins fail to be upregulated in Dicerfl/fl Dhh-Cre+ nerves, with expression levels of Oct6, Egr2, and Sox10 in Dicerfl/fl Dhh-Cre+ p4 nerves closely matching those in Dicerwt/fl Dhh-Cre+ E17 nerves.
Developing peripheral nerves induce expression of NGF in their targets during embryo development while upregulation of NGF expression by Schwann cells following nerve injury is thought to facilitate growth of regenerating peripheral axons back toward their original targets [3].
In the sciatic nerves, the expression of inducible nitric oxide synthase, cyclooxygenase-2 and nuclear factor kappa B was reduced.
Together, the highly limb-specific and nerve-dependent expression patterns of the keratin genes strongly suggest that they are integral to the formation of the blastema.
Most recently, nerve-dependent expression of the transcription factor sp9 has been identified as an early marker of dedifferentiation of the wound epithelium and the initiation of limb regeneration [ 11].
It was shown that the electrical stimulation of the vagus nerve induces the expression of brain IL-1, and vagotomy abrogates the induction of expression of brain IL-1 in response to intraperitoneal lipopolysaccharide [ 14, 15].
Monolateral CCI of sciatic nerve increased GFAP expression mainly in the gray matter of sensory cortex and decreased NFP expression as a consequence of nerve damage.
