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We determined that the cells undergoing apoptosis were neurons by double immunostaining for cleaved caspase 3 and the neuronal marker NeuN (Supplementary Fig. 3b) as well as their anatomical location in nerve cell layers of cortex and hippocampus.
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Increased cellularity in the nerve fiber/ganglion cell layers, enhanced glial fibrillary acidic protein expression, and elevated superoxide level were found only in hypertensive retinas.
Spatial variations in the thickness of the nerve fiber and ganglion cell layers were mapped by segmenting and measuring the layer thickness with a semi-automatic segmentation algorithm.
The molecule is widely distributed in healthy membrane, in the lamina propria, in vessel structures, nerves and smooth-muscle cell layers [ 3].
Nerve cell numbers were counted in layers I III of the cerebral cortex and in the pontine nucleus.
Large numbers of NPs and AT8-ir nerve cells appear in layers II and IIIab.
Segmentation measurements performed at each scan location are illustrated in figure 1. Delineations include thicknesses of the nerve fibre layer/ganglion cell layer (NFL/GCL), inner plexiform layer (IPL), inner nuclear layer/outer plexiform layer (INL/OPL), outer nuclear layer (ONL) and photoreceptor layers together with the retinal pigment epithelium (PR/RPE).
A nasal biopsy from the seventh participant (who also saw improvement but had to drop out of the study due to thalidomide-induced nerve damage) showed more smooth muscle cell layers than were present in samples from untreated HHT patients.
NOS activity could not be detected in the mucus, sensory and nerve cell-rich epidermal layer, therefore NOS is unlikely to be involved in the coral's sensory or nervous system as demonstrated for some asymbiotic cnidarians such as the jellyfish Aglantha digitale [3] and the hydroid Hydra viridis [2].
Although systemic administration of aldosterone caused RGC loss associated with thinning of the retinal nerve fiber layer without elevated IOP, the other cell layers appeared to be unaffected.
The optic tract fibres make synapses with nerve cells in the respective layers of the lateral geniculate body, and the axons of these third-order nerve cells pass upward to the calcarine fissure (a furrow) in each occipital lobe of the cerebral cortex.
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