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Where Neonatal is a binary variable equal to 1 if the child died in the neonate stage and zero otherwise.
Finally, we sacrificed 3 mice from different litters, from neonate stage (day +1) to adult stage (day +50) each two days, to perform the quantification of antithrombin mRNA levels and miRNAs miR-18a and miR-19b levels, during post-natal development.
Corn earworm (Helicoverpa zea Boddie) larvae (neonate stage) were fed on fresh silks (Additional file 1: Figure S1) collected from the same Pr1 and pr1 plants that were used for HPLC analysis.
Under these conditions and depending on feeding regimen, amictic B. manjavacas females hatched from amictic eggs have a prereproductive (neonate) stage of 1 2 days, reach maximum reproductive output at day 4 6, produce 22 32 eggs and live an average of 8 12 days (Gribble & Mark Welch, 2013).
The results herein indicate that the inhibitory effects of gossypol on H. armigera can be observed even if the insect is exposed later in its development, since we did not rear the insects on the gossypol-containing diet from the neonate stage as done by other researchers [ 13, 25, 32].
Under our culture conditions and depending on feeding regimen, amictic B. manjavacas females hatched from amictic eggs have a prereproductive (neonate) stage of 1 2 days, reach maximum reproductive output at day 4 6, produce 22 32 eggs and live an average of 8 12 days (Gribble & Mark Welch, 2013).
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Small babies (preterm, small for gestational age or both) and infants of multiple gestation had a higher likelihood of dying in the neonatal stage indicating needs to provide essential neonatal care to this vulnerable group of neonates.
Therefore, in triplet litters one neonate was euthanized on postnatal day 2 and these subjects were used to investigate the effects of prenatal DEX at the neonatal stage.
Neonates were staged as "P0" on their birthday.
Radical treatment during neonate or infant stage is required for these patients, and gene therapy is a promising candidate for such treatment.
For each gene, the effects of prenatal DEX treatment were first studied in a mixed-model ANOVA that included developmental stage (neonate, adult) as a between-subjects factor.
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