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As expected, more wDi transcripts were detected in nematode tissues known to contain Wolbachia (FBW, FH which includes body wall tissue, FU and MBW, Table 2).
Interestingly, some Wolbachia genes upregulated in nematode tissues include transport systems and genes predicted to be involved in the symbiotic relationship with D. immitis.
Furthermore, the presence of Wolbachia transcripts and proteins in D. immitis tissues lacking Wolbachia suggests that the symbiont transcripts and proteins may traffic to other nematode tissues.
Such spatial resolution of gene expression can provide greater understanding not only on how that specific tissue may interact with the vertebrate host (or evade the host immune system), but also how Wolbachia may differentially contribute to various nematode tissues.
These data provide the first reported insight into the tissue and subcellular localization of nematode CBS-1 at the protein level and indicate which nematode tissues can metabolize homocysteine to cystathionine and/or cysteine to hydrogen sulfide.
Exactly how these transcripts were detected in a tissue shown to be completely devoid of Wolbachia [ 5] remains unclear; however, it is possible these Wolbachia transcripts were exported from other nematode tissues.
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The isolation of mitochondria from nematode tissue requires harsh treatments to break the tough cuticle.
For that study, frozen nematode tissue was disrupted with frozen zirconia beads (BioSpec Products, Bartlesville, OK) in a beadbeater (BioSpec Products, Bartlesville, OK).
Clarification of functions with this significance in the comprehensive manner has not been previously accomplished and reported for any nematode tissue or organ.
We therefore implemented parallel transcriptomic and proteomic analysis to study filarial nematode tissue-specific transcriptional patterns in order to gain functional information.
The airbrush was moved right to left, top to bottom, and vice versa, for around 1200 times, while maintaining a distance of 10 cm between the nozzle and nematode tissue surfaces.
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