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Acytokinetic nuclear division was then observed in young feeding cells which presented a dense cytoplasm, identified as newly induced GCs close to the nematode head (Figure 1C,C').
In Fig. 8, forward motion displays curvature lines with a positive slope (left and middle column); waves are initiated at the nematode head [13], [18].
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In the WT strain, there was abundant attachment of Y. pestis biofilms on nematode heads, allowing only a small portion (about 25%) of larvae to develop into L4/adult nematodes.
Trajectories swept by the nematode tail (or head) are labeled, illustrating striking differences in the travel paths adopted by C. elegans as a function of the surrounding environment.
As shown in Fig. 1, in wild-type nematodes, no obvious alterations of head thrash were recorded in nematodes treated with heat-shock for 0.5 h.
To assay the head thrashes, nematodes were washed with the double-distilled water, followed by washing with modified K medium.
This may yield better segmentation results, in particular at the nematode extremities (i.e. head and tail).
In 10 and 20 µM KCN movement was slower and nematodes were curled, that is, head and tail were in close proximity resembling the conformation that we have previously observed as nematodes reach the root surface [6].
One clue is that some chemosensory neurons located in the anterior head region of the nematode i.e. the amphidial chemoreceptive neurons, dye fill in C. elegans [11], animal parasitic nematodes [12] and cyst nematodes [2].
Utilizing a transgenic approach to visualize CREB-dependent transcription in vivo, we show that this CaMK cascade regulates CRE-mediated transcription in a subset of head neurons in living nematodes.
For example, cases where the nematode coils on itself, or when its head and tail touch (e.g. omega bend), are currently not supported with the implemented skeleton algorithm.
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