Exact(11)
The inference of AS is confounded somewhat by the variable hybridization intensities of neighboring probe sets within a sample and differential gene expression between samples.
Based on the copy number measurements for all target sequences, the breakpoints were estimated to be located between two neighboring probe target sites showing different copy numbers.
Maximum distance for two probes to be considered as neighbors is 1000 bp, a probe is considered "bound" if P Xbar) < 0.001 and central probe has P X) < 0.001 and at least one neighboring probe has P X) < 0.1.
By first analyzing the data solely on the basis of neighboring probe similarity as opposed to signal averages within EcoR1 fragments, the effect of outliers within restriction fragments that contain only a single array probe is ablated.
An approach developed for bacterial CGH, implemented in a program called TSTEP, identifies deletions using microarray data by scoring probes as absent or present based on perfect match and mismatched probe intensities as well as consideration of neighboring probe data using a sliding window [ 20].
A "probe weight" is calculated for each probe sphere of the final set comprising the burial count and the number of neighboring probe spheres.
Similar(49)
The virtual probes without LOH information are assigned the average value of neighboring probes with information.
This explicitly correlates neighboring probes on a segment with similar copy-number and approximates structural changes in the genome.
The poly-G effect seems to be related to the crowded arrangement of probes which facilitates complex formation of neighboring probes with at minimum three adjacent G's in their sequence.
The hypothesis is that DNA immobilized onto nanostructured surfaces reduces steric hindrance from neighboring probes.
The F-tests were calculated with the James-Stein shrinkage estimate using information from neighboring probes [ 43].
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