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The assemblies produced by running Velvet (k=31 and k=55) on simulated, perfect reads generated from the reference E.coli genome (200 × coverage) had only a negligible number of errors (less than 10 for k=31 and less than 5 for k=55).
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A non-negligible number of errors are generated during PCR [ 42] and pyrosequencing [ 37].
One consideration with standard next-gen assembly software is that too much coverage can actually harm assembly quality, that is, extremely high coverage can introduce a non-negligible number of errors into assemblies and assembly quality can suffer, or at least not continue to improve (Haridas et al. 2011; Lin et al. 2011).
"So you see, in reality, a negligible number of Jews were arrested," Roginsky said.
She also criticised the negligible number of council homes being built in London.
The rest are adaptives, with a negligible number of home-centred ones.
A negligible number of HIV-infected children were on ART.
Only in a negligible number of cases was an interview denied.
A negligible number of participants had missing information for most covariates.
A negligible number of hits were found when Vgt1 N28 was the reference.
In contrast, splenocytes from mice immunized with pVAX1 presented negligible numbers of IFN-γ secreting cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com