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An additional cut-off threshold of twofold change in gene expression (either upregulation or downregulation) was used to characterize a gene as being differentially regulated (for example, a negative twofold value corresponded to a twofold downregulation).
The reduction of cytochrome b6f complex (negative fivefold), ATP synthases (negative twofold), and photosystem II (PSII) proteins may explain at least partially the observed reduction in photosynthesis, confirming the observations of Majeran et al. [ 52], which showed a degradation of cytochrome b6f and light harvesting complexes (LHCs) after N starvation.
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The negative answer was twofold.
However, one can observe that for low enough negative ion temperature, twofold behavior of the space-charge emerges.
The negative factors were twofold: too much so-so material, hoovered up from Hong Kong dealers, and good material that was sometimes overestimated.
The twofold negative charge of the ligand is achieved by deprotonation of carboxylic group and secondary hydroxyl group [ 34].
Instead, the data are much better described by a model in which each TOG domain in TOG1-TOG2 independentlyependently with its αβ-tubulin (the best fit was obtained from a model invoking less than twofold negative cooperativity, not shown).
The TCF/LEF signature consisted of 74 genes that were responsive to dominant-negative TCF-4 (twofold cut-off) in colon cancer cells.
Indeed, varanid NCX density at negative membrane potentials is twofold higher than the turtle (15).
To evaluate analytical sensitivity, a small subset of sera from the field collection (n = 3) and all the sera from the experimentally infected animals were also serially diluted (twofold) in negative serum, and tested with the procedure described above.
He said the government's campaign against Web-based expression seemed to have a twofold purpose: preventing negative news from reaching the outside world and preventing Uighurs from sharing such news — or government criticism — with one another.
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