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False negative transcripts are present in expressed transcripts that cannot be matched with a transcript from the estimated transcript set.
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Wiggle plots for transcripts that are expressed from either the positive or negative transcript strand are displayed above (positive strand transcription) or below (negative strand transcription) the central line.
Blue and Red squares were denoted increased (positive) and decreased (negative) transcript abundances (TAs), respectively.
The biological processing and negative transcript regulatory function of miR have been well characterised and reviewed (Ameres and Zamore, 2013).
All these results indicate that GeneScissors is effective in detecting and correcting false positive and false negative transcript reports caused by fragment misalignments.
A breakdown of the results by positive (transcript over-expressed in forager compared to one-day-old honey bees) and negative (transcript over-expressed in one-day-old compared to forager honey bees) significant differential expression is provided in Additional file 1.
We used antisense morpholino technology to generate zebrafish embryos with dominant-negative transcripts of col6a1 that result in severe morphologic, structural and functional changes.
The aim of this study was to investigate the role of rs4072037 in relation to the alternative splicing events of MUC1, in both TR-containing and TR-negative transcripts, for a range of normal non-cancer tissues, to help evaluate published results and as a platform for future cancer studies.
Moreover, knockdown of TRPM2-TE (proposed as a dominant-negative transcript) increased the vulnerability of melanoma cells to undergo apoptosis and necrosis, and overexpression of wild-type TRPM2 in melanoma cells leads to a faster proliferation [ 38, 44].
Strand specific RNAseq data is also supported by RNASeqBrowser that displays reads above (positive strand transcript) or below (negative strand transcripts) a central line.
Both the presence and extent of A-to-I A-to-I A-to-Inegative RNA transitests suggest a negatine correlation between m6A-negative-I.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com