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Wiggle plots for transcripts that are expressed from either the positive or negative transcript strand are displayed above (positive strand transcription) or below (negative strand transcription) the central line.
Blue and Red squares were denoted increased (positive) and decreased (negative) transcript abundances (TAs), respectively.
The biological processing and negative transcript regulatory function of miR have been well characterised and reviewed (Ameres and Zamore, 2013).
All these results indicate that GeneScissors is effective in detecting and correcting false positive and false negative transcript reports caused by fragment misalignments.
A breakdown of the results by positive (transcript over-expressed in forager compared to one-day-old honey bees) and negative (transcript over-expressed in one-day-old compared to forager honey bees) significant differential expression is provided in Additional file 1.
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False negative transcripts are present in expressed transcripts that cannot be matched with a transcript from the estimated transcript set.
Moreover, knockdown of TRPM2-TE (proposed as a dominant-negative transcript) increased the vulnerability of melanoma cells to undergo apoptosis and necrosis, and overexpression of wild-type TRPM2 in melanoma cells leads to a faster proliferation [ 38, 44].
We used antisense morpholino technology to generate zebrafish embryos with dominant-negative transcripts of col6a1 that result in severe morphologic, structural and functional changes.
The aim of this study was to investigate the role of rs4072037 in relation to the alternative splicing events of MUC1, in both TR-containing and TR-negative transcripts, for a range of normal non-cancer tissues, to help evaluate published results and as a platform for future cancer studies.
(A ) Schematic shows methodology for identifying motifs enriched in the 3′ UTR regions (500-nt) of transcripts increased or stabilized in torpor (positive set; transcripts in DIANA Clusters 4 6) compared to transcripts decreased in torpor (negative set; transcripts in DIANA Clusters 1 3).
Background was modeled for each mass of RNA as the average of negative control transcript counts plus three standard deviations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com