Our study demonstrates distinct profiles of TNF-α-activated gene expression in Thy-1 positive (Thy-1+) and negative (Thy-1−) subsets of mouse embryonic fibroblasts (MEF).
Three districts in the northern part of Jutland were negative (Fussingø, Thy, and Ulborg; Figure 1), but only 9 blood samples came from these 3 districts.
In this context, it was shown that fibroblasts in IPF lungs are Thy-1 negative, whereas most fibroblasts from normal lungs are Thy-1 positive [ 37].
For example, human fibroblasts that express the cell surface antigen Thy-1 are capable of TGF-β1 stimulated myofibroblast differentiation, while Thy-1 negative fibroblasts appear to be only capable of lipofibroblast differentiation [ 42].
The parental BW5147.3 and the Thy-1 negative, DPM1-deficient, mouse lymphoma cell lines [24], [25] (ATCC, Rockville, MD) and EBV-transformed cell lines were cultivated in RPMI 1640 Glutamax™ medium containing 10% fetal calf serum and 1% penicillin/streptomycin at 37°C under an atmosphere containing 5% CO2.
Subsequently, specific antibody-binding capacity was obtained after corrections for background, apparent antibody-binding capacity of the negative control mAb anti-Thy-1.2.
Mice injected intramuscularly with either subset had enlarged spleens by the second week of tumour growth caused largely by the accumulation of Ig, Lyt-1 and Thy-1 negative cells.
The stem cell markers c-KIT and THY-1 were negative.
Although the stem cell markers c-Kit and THY-1 were negative, a strong positivity was found for OCT-4.
The tagline of this year's The Crazies, "Fear thy neighbor", is probably as negative a message about mental illness as has ever made it to a cinema poster.
Studies have established that SSCs express α6- and β1-integrin, GFRα1, CD9, Thy-1, and EpCAM but are negative for c-kit (Kit) or SSEA-1 [3].
