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Exact(7)
Activated basophils were identified as CD63high and cut-off was set with the negative stimulation control.
In contrast, the deflection during negative stimulation was 100% ±1%of the control level (56 pulses analyzed).
Samples were considered positive at activation percentage >5% CD63high cells and stimulation index (SI; % activated basophils divided by % activated basophils of the negative stimulation control) >2.
Sulfidoleukotrienes were considered significantly increased when above 163.8 pg/ml, a cut-off that was calculated from the mean plus 3*SD of negative stimulation controls (patient #54 excluded because of high background).
Delivery of the fetus can early avoid excessive negative stimulation to fetus by the acute raised IAP, and might decrease the perinatal mortality.
For this purpose, A 100 μl of each concentrations of extracts and negative (stimulation buffer)/positive controls (anti-FcϵR1 monoclonal antibody, fMLP) were added into the sample tubes.
Similar(52)
Negative (no stimulation) and positive (10 µM ionomycin) controls were included in all experiments.
Separate patches were tested for positive and negative pressure stimulation.
Typically the onset of responses was approx. 2 4 ms following a negative DC stimulation.
To conclusively determine whether this Ca2+ influx was Piezo1-mediated, we subjected Vav1-P1cKO RBCs to similar negative pressure stimulation.
10.7554/eLife.12088.005 Figure 2. Mean Piezo1 current responses for all patch configurations upon positive and negative pressure stimulation.
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