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The negative round of selection was carried out with a plasmid encoding a toxic barnase gene with amber mutations at three permissive sites (Gln2TAG, Asp44TAG, and Gly65TAG) in the absence of 3-Me-His.
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Twelve rounds of positive and eleven rounds of negative rounds of negative selection were performed to identify the candidate ssDNA MRE.
The surviving AcPheRS variants from two positive and one negative rounds of selection were subsequently screened for chloramphenicol resistance level in the presence and absence of AcPhe.
The negative rounds of selection in which aptamer candidates displaying affinity for the free GST affinity tag were purged from the DNA pool seem to have been successful, as indicated by these results.
Prebiopsy MRI was first proposed in patients after a negative first round of biopsies.
Two more rounds of panning were performed in this manner, with decreasing incubation times for positive rounds and increasing incubation times for negative rounds.
This assumption was made after observing that 95% of those testing HIV negative in a particular round of serological survey remained negative in the ensuing 5 years.
They worried that a second round of negative reviews could be fatal.
And it was the president's decision to start an early round of negative ads against Mr. Kerry, who with the other Democratic candidates had been lobbing verbal explosives at Mr. Bush from the first of the year.
The announcement, made on her daytime talk show, came as a surprise to Broadway insiders, many of whom had written off "Seussical" when it received a round of negative reviews after opening on Nov. 30.
After receiving a round of negative reviews, "Jackie Mason's Laughing Room Only," a new musical comedy revue at the Brooks Atkinson Theater, will close on Nov. 30, Mr. Mason's spokesman said yesterday.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com