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The mechanisms underlying this are not clear, but the regulation of Bcl-w protein levels is unlikely mediated by the ubiquitin-proteasome pathway, as evidenced by the negative result obtained with a proteasome inhibitor.
The negative result obtained for patient 8 might suggest that some MCC patients are not infected with MCPyV.
A true negative result was defined as a negative result obtained by cell culture, and at least one of the other two methods.
The negative result obtained from trigeminal ganglion belonging to animal 199 could be due to technical problems with conservation and/or processing of the sample.
Knowledge of the genotypes of the tested animals is necessary to interpret results of diagnostic tests: a negative result obtained from a genetically susceptible animal brings more evidence of scrapie freedom than a negative result obtained from a genetically resistant animal (because even if the flock were infected, this animal would probably have remained negative).
The negative result obtained by PCR of CSF from patient 4 could have been the consequence of inappropriate storage of the sample at high room temperature for 72 hours before analysis.
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Negative results obtained with RNA from four other fish viruses confirmed the specificity of the test.
The rates of concordance of positive or negative results obtained following immediate incubation and following 6- and 12-h delays were 77.8% (14/18) and 79.4% (27/34), respectively.
But the negative results obtained with the first 134 patients have already led the Health Ministry to pull them off the treatment, as well as those who were getting state-supported treatment for the same protocols and whose symptoms have worsened.
The designed IPC assay has proven to be an effective tool for monitoring inhibitors of PCR and builds confidence in negative results obtained with agent specific assays.
In addition, the negative results obtained with the life-detecting instruments onboard the 1976 Viking landers40 may find the simplest explanation in the fact that, in both the Gas Exchange and Labeled Release experiments, samples were incubated with various watery solutions41 with high water activities.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com