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More importantly, this strain of staphylococcus was coagulase negative, resistant to novobiocin but sensitive to Polymycin B, which differentiated S. sciuri from S. hyicus (Table 2).
Gram negative resistant organisms were sensitive to other beta-lactam antibiotics; five of these patients were treated with piperacillin tazobactam and 2 received imepenam (carbepenam group) as per treating physician's decision.
Both isolates were sent to MORU for confirmation, where they were found to have typical colony morphologies on Ashdown's agar (purple colour, metallic sheen, becoming dry and wrinkled after 48 hours), gave positive results to oxidase and latex agglutination test, L-arabinose negative, resistant to gentamicin and colistin.
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In the intensive care unit (ICU), Gram-negative resistant pathogens are responsible for longer hospitalizations and poorer outcomes [2, 3].
A confluent bacterial growth on MacConkey agar was obtained from all studied animals, revealing the constant presence of non-fastidious facultative or aerobic gram-negatives resistant to bile salts.
Gram-negative resistant strains predominate among our ICU bacterial population.
The simplified SDD protocol reduced ICU acquisition of Gram-negative resistant infections without a rise in Gram-positives or fungi.
Third-generation cephalosporin-resistant K. pneumoniae was the predominant gram-negative resistant pathogen, with an incidence density of 0.19/1,000 and 3.21/1,000 inpatient days for blood and ICU cultures, respectively.
We identified 66 studies with interpretable data, of which 16 reported 20 microbiologic outcomes: gram-negative resistant bacteria, 10 studies; Clostridium difficile associated diarrhea, 5 studies; vancomycin-resistant enterococci, 3 studies; and methicillin-resistant Staphylococcus aureus, 2 studies.
Therefore, we were more interested in factors associated with resistance to our usual antibacterial first-line therapy, rather than in factors associated with ESBLs, and deliberately chose to develop a score combining both Gram-positive and Gram-negative resistant bacteria.
Spread of gram-negative resistant bacteria from the hospital to the environment by direct person-to-person contact or through nonsanitized water is a concern in under-resourced countries (19 ).
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