There is also an additional negative principle, to the effect that the model itself should not be designed to include dependencies on characteristics relating to the configuration or properties of an individual piece of equipment.
Although the detailed biologies of the two systems are very different, the unifying feature of both studies is the use of negative design principles.
However, we show that the presence of the remainder of the sequence dramatically reduces the net overall aggregation propensity by negative design principles that we suggest are very common in biological systems as a result of evolutionary processes.
It has been suggested that evolution exploits negative design principles to modulate protein deposition by placing residues that counteract aggregation at the flanks of hot spots [21], [27], [64].
We could confirm that nature uses negative design principles to avoid the self-assembly of aggregation-prone regions in globular cytosolic proteins as well as the strongly decreased aggregation propensity of cytosolic IUPs, as previously proposed by Serrano and Chiti groups by analyzing different virtual proteomes [21], [22], [27].
A widely accepted evolutionary hypothesis is that proteins have utilized negative design principles to generate sequences that can not only reach the final desired structure but can also avoid unproductive pathways (3, 10, 14).
Commenting on the ban on the Foreign Affairs Committee entering Hong Kong, Mr Graham said it was "sad" China had concluded that the committee's report, which has not yet been drafted, must be "negative in principle because of its existence rather than its content".
MicroRNA regulation of viral gene expression and replication exploits the negative regulatory principle of microRNA mRNA degradation.
In-gel SOD activity was visualized using the NBT (Nitro Blue Tetrazolium) negative staining principle of Beauchamp and Fridovich [ 53].
Blood glucose level was clamped at 5.5 mmol/l using a variable infusion of 20% glucose, based on the negative feedback principle (14).
