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For the purpose of first assigning the peptide determinants reactive with patient serum, all ninety five peptides provided by Synpep were tested for the positive and negative pool of sera as described above.
Once a negative pool is chosen, 50 classifiers are constructed for each TF using different random subsamples from the negative pool.
This is done by randomly under-sampling from the negative pool.
All these results indicate a tendency for false negative pool detection rather than for false positive pool detection.
Since the negative pool is expected to be noisy, the repeat sampling assures that no single, biased gene group dominates the classifier.
Region B represented a third category, where the positive pool contained <10% CBS1502 alleles, while the negative pool contained ∼50% CBS1502 alleles.
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First-passage negative pools were passaged a second time to confirm absence of avian influenza.
GFP positive F1 or F2 fish were outcrossed, and embryos were sorted into GFP positive and GFP negative pools.
RNA was recovered from the sorted, p16-positive/p21-negative and control (p16-negative/p21 negative) pools of cells and subjected to qPCR and microarray analysis.
The data (not shown) confirmed that these four regions segregate nonrandomly in the positive and negative pools as predicted by the sequence data.
Of the 51 negative pools, two included one positive animal, and they were both only positive in one of the two tests.
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CEO of Professional Science Editing for Scientists @ prosciediting.com