Exact(8)
Fifty-one positive and one negative milk sample was determined by commercial assay.
each were constructed, they contained 10 100 % BVDV antibody positive milk, diluted in antibody negative milk.
BVDV antibody negative milk and serum samples used for the dilutions were tested negative in both ELISAs.
We did not have enough negative milk or saliva samples available for us to determine the cut-off values for our milk and saliva assays.
A test setup was considered valid if the negative milk control had an OD below 0.2 and the weak positive milk control had OD between 0.6 and 1.0.
In the clinical mastitis cases, there were a higher proportion of bacteriological negative milk samples from organic farms (30.9% versus 18.1%).
Milk and serum samples from these ten lactating cows were serially two-fold diluted in seven dilution steps from 1/2 up to 1/128 in BVDV antibody negative milk or serum, respectively.
Four cows were kept for the study based on these criteria: no calving associated problem and bacteriologically negative milk samples with SCC values below 1 × 105 cells/mL across lactation.
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