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For Principal Component 1 in this study, several of the relatively strong positive loaders (serine, glycine, alanine, aspartate) are fairly close in metabolic space to glutamate, which is a relatively strong negative loader, and thus somewhat distant from them in our metabolite space.
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The biomarkers present in the tricarboxylic acid cycle (malate, succinate and citrate) are all strong negative loaders on Principal Component 2. This component tends to vary more with days post-emergence than with the more strictly developmental variable of height of sampling of the tissue.
A number of the involved amino acids were also relatively strong – positive or negative – contributors (loaders) on the Principal Component 1 of our study.
The loader, The docker, the navvy.
Nowadays, the Michigan loader is retired.
Quemada-Diez became his clapper loader.
Negative, negative, negative.
I was a loader, I had to load.
He opened the door of his loader and screamed.
Greg works as a loader in a bulk-goods warehouse.
"Look at that front-end loader," he said admiringly.
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