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However, negative host plant effects, might be overcome through directed selection.
Perforation or delamination of the coatings can expose the substrate to accelerated wear or corrosion, and the released coating particles may act to accelerate third party wear resulting in a negative host response.
Taken together, our expression system based on the cDNA1- and tef1-driven expression vectors and the cellulase negative host strain Δ xyr1 is suitable for a high-throughput system for testing of cellulase muteins.
Alternatively, if the interaction of P66 with β3 chain integrins serves to down-regulate a negative host response to the bacteria, in the absence of β3 chain integrins, the presence of P66 should not be required.
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Specificity was calculated as the fraction of actual negative [true negative (TN)] host samples divided by all expected negative hosts including both unexpected positive [false positive (FP)] and TN, as follows: Specificity = TN/ FP + TN).
However, this construct displayed host specific activity in other Gram negative hosts that was consistent with the RT-PCR results.
All Gram negative hosts containing plasmid R995 gave the expected negative results in the RT-PCR analysis.
A series of broad-host-range vectors carrying a promoterless lacZYA operon was constructed to test promoter activity in a wide range of Gram negative hosts.
We then transferred these constructs to other Gram negative hosts and assayed lacZ expression in each host compared to a vector only control.
We transferred R995 + island 4305 to ten other Gram negative hosts belonging to eight different genera as described in the Materials and Methods.
To our knowledge, this is the first systematic analysis of horizontally-transferred genomic island gene expression in a broad range of Gram negative hosts.
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