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Furthermore, the lack of a true negative fraction did not allow the specificity to be determined.
To resolve this critical issue, we designed a study where an immuno-magnetic CD138 enriched positive selection was used for FISH while the negative fraction (NF) was used to retrieve other myeloid elements for RCA.
The ROC curve technique is based on plotting model sensitivity, true positive fraction values calculated for different threshold values versus model specificity, true negative fraction values on a graph (Deleo 1993).
The negative fraction was allowed to flow through and collected for further analysis.
In each experiment, a STRO-1 negative fraction was treated accordingly.
Similar results were found when analyzing expression in sorted PSA-NCAM+ cells by comparison to the negative fraction.
Similar(19)
Analysis of the Oct4ΔPE GFP-negative fractiOct4ΔPE GFP-negativee lines at day 35 supports this suggestion (data not shown).
The "CD133+" fraction contained 92.2% CD133-positive cells, whereas the CD133- negative" fraCD133- negative 5.4% ofractionpositive contained
B cells present in the CD11c-negative fraction were labelled with PerCP-conjugated CD45R/B220 (clone RA3-6B2, BD Pharmingen).
Subsequently, the CD19-negative fraction was FACS sorted (MoFlo™, Dako) using CD4-FITC (Caltag, RM2501) and CD62L-phycoerythrin antibodies (Caltag, RM4304) to purify CD4hi, CD62Lhi population.
At this value, the true-positive fraction is 72.7%, with a true-negative fraction of 53.3%.
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