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Richardson, J.S. & Richardson, D.C. Natural β-sheet proteins use negative design to avoid edge-to-edge aggregation.
Such evolutionary bottleneck is overcome using the negative design evolutionary pressure applied against promiscuous homodimer formation.
These results suggest that extensive negative design is not required to create well-folded β sandwich proteins.
These results demonstrate the robustness of the ORBIT protein design methods and underscore the need for continued improvements in negative design.
The most potent inhibitors included both positive and negative design elements and exploited interaction with the coiled-coil and basic DNA-binding regions of BZLF1.
However, it is important to note that negative design elements may be encoded in the conformation of the protein backbone which was preserved from the wild-type protein.
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The recently developed test-negative design is now standard for observational studies of influenza vaccine effectiveness (VE).
Lewnard JA, Tedijanto C, Cowling BJ, Lipsitch M. Measurement of vaccine direct effects under the test-negative design.
The test-negative design (TND) has emerged as a simple method for evaluating vaccine effectiveness (VE).
Observational studies of influenza vaccine effectiveness (VE) are increasingly using the test-negative design.
The test-negative design (TND) case-control study is commonly used to obtain IVE estimates.
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