Based on these data, we performed a ROC analysis using the QFT-IT-negative cured TB subjects and the healthy controls as comparator groups.
The number of PCR-negative (cured) animals fell to 4 of 9, indicating that some low-level infections only become detectable by PCR after immunosuppression.
Non-stretched polyimide films based on 5,4′-diamino-2-phenyl benzimidazole (DAPBI) show curious thermal expansion properties: the in-plane CTE value of PI film is negative when cured at 350 °C (contract upon heating).
We subsequently confirmed that these new populations (LHM-W and LHM-bw-W-, W- for Wolbachia negative) were cured from Wolbachia infection, again using PCR (data not shown).
As control groups, we enrolled: 1) "controls", individuals with no risk of M. tuberculosis infection who tested TST-negative and QFT-IT-negative; 2) "cured tuberculosis", individuals with culture-positive pulmonary tuberculosis at the time of diagnosis and who were culture-negative upon treatment completion when the draw blood was performed.
The recent report that allogeneic HSC transplantation with naturally-resistant CCR5-negative HSCs cured a patient with AIDS [10] lends support to this approach as a viable therapeutic option, at least until an effective vaccine can be developed.
Whether they are still culture-positive while being smear-negative or temporarily "cured" (i.e. culture-negative) is largely unknown.
According to the results of the performed tests at the end of follow-up, cases were identified as cured (negative follow-up) or relapsed (positive histology).
SD, standard deviation; EPTB, extra-pulmonary tuberculosis ; PTB+, smear positive pulmonary tuberculosis ; PTB-, smear negative pulmonary tuberculosis; cured + completed = therapeutic success.
The first report for inconsistent positive tissue PCR in mice considered cured by negative conventional serology, hemoculture, and PCR was documented [ 50].
Two other cured (culture-negative) patients later died (1 of a narcotic overdose and the other of respiratory insufficiency).
