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This process was then repeated 25 times and the positive and negative coordination values were used to estimate the mean and standard deviation of positive and negative coordination for each network.
Next, we calculated positive and negative coordination values between each HCG and the entire set of genes in the genome.
Moreover, our results showing high positive coordination with no negative coordination among HCGs of the same group and high negative coordination with no positive coordination between HCGs of different groups within the same network imply that our Gene Coordination approach can identify HCGs groups and networks without any prior experimental knowledge for their existence.
Last, we considered a gene to be highly positively or negatively coordinated with a given group of the HCGs if it had a statistically significant positive or negative coordination with all members of that HCGs group.
To identify the HCGs within each network, we used a coordination matrix (using heat map representation) in which the upper right triangle shows positive coordination and the lower left triangle shows negative coordination (Fig. 3).
Notably, most of the HCGs of the two networks exhibited both a considerable cross-network negative coordination (panel A) as well as a considerable cross-network positive coordination (panel B) between them under different biological perturbations.
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In order to identify the HCGs within each network, we used a background model for the evaluation of the negative and positive coordination in each network, assuming no coordination, and considered genes to be HCGs if their observed coordination was higher by more then six standard deviations from the coordination that was estimated by our background model (for detail see Methods section).
We considered genes as HCGs if they exhibit either negative or positive coordination, which was more then six standard deviation above the background coordination.
In spite of its negative roles the coordination chemistry of Pb II) complexes is a matter of interest.
Stern and El-Ansary (1992) supported the statement that informational power is likely to have a negative effect on coordination in channels of distribution.
In spite of its negative roles the coordination chemistry of Pb II) complexes is a matter of interest, which display interesting structural features as a consequence of the large radius, adoption of different coordination numbers from 2 to 10 and especially based on the extent to which the lone pair is stereo chemically active [11 15].
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