Contamination with Gram-positive bacteria was more common; however, Gram-negative contamination was independently associated with increased risk for death.
This model was originally developed with a mixed Gram-positive/Gram-negative contamination and was modified in this study to include ABC and MRSA to more accurately reflect the clinical condition outlined above.
All DNA extraction and PCR blanks were negative for contamination (Table S4 ).
Negative mycoplasma contamination status was verified using LookOut Mycoplasma PCR Kit (Sigma) and MycoAlert Mycoplasma Detection Kit plus Assay Control (Lonza).
All cell lines were tested negative for contamination with Mycoplasma spp. and maintained in a controlled environment of 5% CO2 and 95% relative humidity at 37°C.
The cell lines tested negative for contamination with Mycoplasma spp and cultured as monolayers in a 95%air/5%% CO2 atmosphere at 37°C.
In order to control nuclease contamination, negative control was included at every reaction.
Samples tested negative for mycoplasmal contamination by both DNA fluorochrome staining and live-culture methods.
To detect possible contamination, negative controls were implemented for each sample for extraction and PCR.
To monitor contamination, negative controls were included during each DNA extraction procedure.
