The challenges were the large velocity range, the lack of contrast, and the large spatial resolution needed to image vessels below 5 mm in diameter.
Therefore, we only needed to image with sufficient resolution to distinguish every cell body throughout the brain.
However, it is possible that, in severe deformity, additional measures may be needed to image the entire region.
If we used traditional MSI to analyze these cell populations, ∼10000000 pixels would have been needed to image the area at a 10 μm lateral resolution.
This limited interaction means that there will be little to no effect on the lipoprotein profile in the time needed to image.
It is thus possible that the degree of defocusing needed to image chromosomes or chromatin with high contrast prevents the visualization of 30-nm chromatin fibers.
Consequently, newborn CAG::mRFP1 pups can be genotyped based on their pink pigmentation, alleviating the need to image them under epifluorescent conditions.
As our projects progressed, we needed to image the molecular concentrations in the early lesion cartilage as directly as possible, at high resolutions.
Based on our results, an estimate of the number of cells needed to image in vivo (μs′ ≈10 cm−1) at a depth of 3 mm is approximately 45 cells.
For instance, the beam power needed to image with 545 nm light in the current AOSLO instrument is about 15 µW.
