Unlike the other methods, chemical modification can unlimitedly alter side chain of amino acid structures without the need of sequence or structure information [11].
In order to unravel how the lineage leading to extant land plants managed (in terms of transcriptional regulation) to become multicellular, we are in need of sequences from other (multicellular) algal genomes that are more closely related to extant land plants, that is, of Charophyta.
NG6 is a complete information system designed to answer the needs of a sequencing platform.
Furthermore, this option cannot be scaled up or down quickly to meet the needs of different sequencing projects.
ORCAE was designed to suit the needs of genome sequencing projects from small consortia, like the grapevine.
The increasing throughput of sequencing raises growing needs for methods of sequence analysis and comparison on a genomic scale, notably, in connection with phylogenetic tree reconstruction.
As a general rule then, the less expensive platform producing the needed amount of sequences for a given project would probably also be the most suitable one, unless platform-specific characteristics (e.g. long reads, usable throughput, etc).
Due to the fact that ( T_{i} ;;i = 1, 2, ldots, n ) in Eq. (6) are in need of a sequence of binary information, a relay, i.e. ( f_{on/off} ) could be realized.
The CGAL is one recent approach that incorporates genome coverage and assembly accuracy into the evaluation without need of reference sequence and combines them into a single metric score (Rahman and Pachter, 2013).
These advances are of particular interest to researchers studying organisms with missing or incomplete genomes, as the need for knowledge of sequence information is overcome.
