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The assays which needs cell lysates, the cell lysate was prepared by treating the cells with TritonX 100 (0.1%; 1 mL) and sonicating the contents for 20 s.
The complex architecture of electrospun scaffolds requires appropriate, for tissue engineering needs, cell seeding and culture methods, to maximize tissue growth in vitro environment.
The ceiling in the RaBIT fermentation system depends on cell maintenance needs, cell biomass yields on substrates, and cell growth/death rate.
Thymic epithelial cell-mediated apoptosis needs cell priming, and 98% of the CD4+CD8+ double-positive immature T lymphocytes undergo apoptosis since they are more sensitive to the BAD, PUMA, and HRK-1 apoptotic sensitizer proteins [ 82] than are single-positive CD4+ or CD8+ cells.
However, the measurement of the fluorescence (yEGFP) can be followed as a function of incubation time and is easier, quicker, and cheaper than the measurement of the β-galactosidase or the luciferase activity, which needs cell wall disruption and/or the addition of expensive substrates.
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In order to develop a new vaccine, Salk needed cell cultures that he could first infect with polio, and then test the treatment.
Simple organisms live short lives and do not need cell division.
My mentors in Benin need cell phones to talk to each other effectively.
However, we need cell biological methods, including detailed location studies, to further characterize such identified candidates.
But the fact is, he needed cells, and we still can't synthesis them.
They also need cells that will remain brain cells and not revert to fat cells or bone cells, he added.
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CEO of Professional Science Editing for Scientists @ prosciediting.com