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Valves are of seven main types: globe, gate, needle, plug (cock), butterfly, poppet, and spool.
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In brief, a hole was punctured in the base of an Eppendorf tube with a 22-gauge needle and plugged with cotton wool.
Other stalls sold things that I'm not totally sure would be neccesary in a survival situation – like acupuncture needles and ear plugs – but people seemed interested in them nonetheless.
We used an Erlenmeyer flask that was branched on the bottom, and a needle (21G) penetrated the culture plug.
Four wells were cut in each agar plate with a heat sterilised cork borer of 6 mm diameter, and the agar plugs removed with a sterile needle.
Each rubber stopper was pierced with a 0.6 mm, Microlance needle (Becton Dickinson, Breda, The Netherlands), fitted with a cotton-wool plugged syringe cylinder to prevent pressure build-up.
The mixture was implanted subcutaneously into two sites at the midline and the needle was retained for 1 2 minutes to allow the plug to solidify.
To account for the platelet plug and locally activated platelets resulting from the needle insertion, the first tube was discarded.
Free the board for removal by disconnecting each wiring harness Take care to separate each plug, making sure to remove each one with needle nose pliers.
The vessel was closed with a silicon plug (AS ONE, Osaka, Japan), into which a hole was bored using a disposable needle (Terumo Corp., Tokyo, Japan).
An Idico filler-plug gun (Forestry Suppliers, Inc., Jackson, MS) equipped with a stainless steel needle similar to that described by Toman and White (1993) was used to deliver the conidial suspension.
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