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A nuclear extract was prepared by MNase digestion to mononucleosomes, followed by needle extraction in a buffer containing either physiological or high salt concentration.
The storage performance of the needle extraction device was also studied.
Optimizing the main experimental parameters for each technique, successful determination of several smoking-related compounds with these needle extraction methods has been demonstrated.
Miniaturized needle extraction device has been developed as a versatile sample preparation device designed for the rapid and simple analysis of smoking-related compounds in smokers' hair samples and environmental tobacco smoke.
Agarose gel image of DNA isolated from soluble and insoluble chromatin after 2 min MNase digestion and needle extraction.
However, use of this ultra thin needle extraction method enabled us to isolate cultivable strains, including Bacillus and Paenibacillus.
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In-needle extraction was applied for preparation of aqueous samples.
A novel in-needle extraction device has been developed for the preconcentration of gaseous organic compounds prior to the determination in gas chromatography (GC).
To determine the content of these VOCs, air samples were collected for 30 min with the packed sorbent(s) in the extraction needle, and the extracted VOCs were thermally desorbed in a GC injection port by the direct insertion of the needle.
This study was intended to investigate the effect of different packing materials on efficacy and reproducibility of VOC analysis by means of needle trap micro extraction (NTME).
Another surgical complication was the breaking of the needle during the extraction procedure from the bone.
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