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After homogenisation by 10 passages through a 22G needle, cells were centrifuged 10 min at 5 000 g at 4°C to eliminate non-homogenized cells.
When the newly formed needle cells start their own photosynthetic assimilation, the assimilate flow associated with the Calvin cycle is insufficient to feed the glycolytic chain with substrates, and the RCP compensates this deficit.
At night, when the Calvin cycle is not activated, the "heavy" photorespiratory carbohydrate flow from old needle cells ceases, whereas the C-depleted "light" flow from the reserve photosynthetic carbohydrate pool (RCP) continues.
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Viscosity was reduced by drawing the suspension through a 21-G needle, cell debris were removed by centrifugation (15 000 g, 10 min), and the supernatant was stored at −80°C.
And this is how he needles cell phone users who have taken to using headsets: "When worn in public, they often make you look 'different' to everyone else -- and not in a good way".
For fine needle aspirates, cells were collected through a 23 gauge needle and then washed.
We have adopted a SMART (Switch Mechanism At the 5' end of RNA Transcript) amplification method [ 14, 15] to amplify cDNA from our needle collection cells.
Undifferentiated GCs were collected from the ovaries by puncturing follicles with a 25-gauge hypodermic needle, and cells were dispensed into 4F medium supplemented with 10 % FBS (GIBCO BRL, Grand Island, NY, USA), and incubated in a humidified atmosphere of 5%% CO2 at 37 °C.
This opening is surrounded by a number of pointed projections called teeth, and Jennings showed that if some of these teeth are experimentally removed with a glass needle, the cell forms a daughter cell with a similarly reduced number of teeth.
In the study of Bang et al., the specimens obtained with the 22G FNA needle underwent cell block analysis for histological assessment, and some studies have shown that cell block is a valid technique for performing histological assessments and can improve the diagnostic accuracy of smears [ 24– 26].
For example, fine-needle biopsies are performed on thyroid masses detected on physical examination or ultrasound imaging, and collections of needle-aspirated cells are viewed by cytopathologists.
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