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If modification to transfection (step 2) is needed, record and maintain modified steps for remaining replicates.
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You needed records!
These new plants came largely to the botanic gardens of Amsterdam, Leiden, Chelsea and Paris and they needed recording.
Each region, in turn, needed records of individual entitlements, such as leave and family allowances.
Gabor [ 38] first introduced the concept of holography, where two steps are needed: recording and reconstruction.
If needed, recorded it on tape then play it back.
Do we even need record companies any more?
Wojciech Szczesny admits Arsenal "need" record goalscorer Thierry Henry back at the club.
In the none-too-distant future, techno-visionaries declare, musicians will not need record labels.
"This was supposedly proof that bands didn't need record companies.
Who needs record stores when you have the Internet?
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