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A larger share of imported vegetables implies a decrease in the thermal energy needed for cultivation under glass and a lower fuel demand for machinery.
The main causative factor for greenhouse gas emissions, acidification and air pollution is the burning of fossil-based fuel for agricultural operations, production of fertilizers and pesticides needed for cultivation (in the case of ethanol), ocean-based transportation (for naphtha) and the chemical processing steps (for all feedstocks).
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The overall process conversion factor of glucose to ethanol from the raw materials was set to 0.80 based on the experimental results after withdrawing the sugar needed for yeast cultivation, since yeast cultivation was not included in the experiments.
> -wrap-foot> Environmental conditions required for ex vitro growth of plants are different from those needed for in vitro cultivation (Hazarika 2003).
Agricultural tools, needed for clearing forests and for cultivation, were the next iron tools to develop.
Primary transformants were selected on 1× MS medium (Sigma), 1% sucrose, 0.7% agar with 20 μg/ml hygromycin or 50 μg/ml kanamycin as needed for 10 days prior to cultivation in soil.
Primary transformants were selected on MS medium (Sigma), 1% sucrose, 0.7% agar with 20 μg/ml hygromycin or 50 μg/ml kanamycin as needed for 10 days prior to cultivation in soil.
Conclusions: These in vitro results demonstrate that thorough investigation of various cultivation conditions is needed for successful expansion and differentiation of stem cells towards different lineages to be used therapeutically for replacement of damaged cells.
Somewhat similar systems are employed with row crops, where chemical weed control assists in reducing need for cultivation.
Modern molecular approaches to detect, characterize, and quantify microorganisms provide rapid and thorough measures unbiased by the need for cultivation.
During the last decade the use of molecular methods have supplied the means for examining microbial diversity and detecting specific organisms without the need for cultivation.
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