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However, they had one inherent limitation: the need to crystallize the enzyme, a laborious procedure requiring enzyme of high purity which meant relatively high costs.
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The idea that became the California Locos first began taking shape in 2002 with Tourjé's solo exhibition Dream Kitchen at the Riverside Art Museum, but it wasn't until 2009, when his friend Gilbert Maguu" Lujan of Los Four told him definitively that more needed to be done to crystallize and articulate the thoughts being cooked up in that kitchen.
It can be seen that a critical block length is needed for components in order to crystallize.
Now, in movie-acting the important, the sensitive moment comes around the third take of a scene; by then you just need a whisper from the director to crystallize it for you.
SE, which formed as a response to the state and market failures in tackling the social problems (Yujuico 2008; Nicholls 2006), needs careful philosophical scrutiny and thought to crystallize the conceptual boundaries and foundations of this phenomenon.
But this may well need an event, or a third party creating an occasion, to crystallize this.
The main disadvantage of CLECs was the need to purify and crystallize the enzyme.
This is particularly the case when specific residues within a certain site or entire regions of a protein need to be altered to change protein activity, stability or ability to crystallize.
"Apple has this unique position in the market where they're able to crystallize a trend that's beginning to crest and educate people on why they need it," he said.
While CDK9/cyclin TQ77R/E96G/F241L crystallizes reliably, attempts to crystallize wild-type cyclin T in complex with CDK9 resulted in very small crystals that proved unsuitable for structural analysis.
In the 1950s, when the borders were still fluid and needing to be crystallized, stories would be brought out to establish that in united India, "Muslims would never have been able to rise up".
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