Sentence examples for need for sequencing from inspiring English sources

As a second step, identification of Alternaria species-groups is obtained through a seminested PCR method, without the need for sequencing PCR products.

Rolling Circle Amplification (RCA) of DNA is a sensitive and cost effective method for the rapid identification of pathogenic fungi without the need for sequencing.

Rolling circle amplification (RCA) of DNA is a sensitive and cost effective method for the rapid identification of pathogens without the need for sequencing.

To develop a reliable tool for the identification and classification of the different Ensifer species, without the need for sequencing, a prototype DNA microarray that targets the rpsA housekeeping gene was designed and tested.

This assay allowed the accurate and rapid detection of MAC bacteremia, distinguishing it from M. tb in a single PCR reaction without any need for sequencing or hybridization protocol to be performed thereafter.

A larger scale study is required to include more hosts to enable development of methods such as PCR-RFLP, which can circumvent the need for sequencing.

This is, however, far from sufficient in view of the important role of insects in diverse ecosystems, great number of insect species affecting human activities as pests or vectors of diseases, and need for sequence data for studies on processes such as insect evolution, ecology, behavior, insecticide resistance, development, and physiology.

The use of a comprehensive infectious agent database allows detection without the need for sequence assembly, however, a limitation of this technique is that that totally novel IA candidates could be missed if their expressed genes are not closely related to any known agent.

This method allows the evaluation of a significant proportion of samples without the need for sequencing- and/or radioactivity-based techniques.

This finding reinforces the need for sequence confirmation of PCR products.

The close localization of CMS genes to syntenic sequence blocks might be due to the need for sequence elements required for transcription of a chimeric orf.