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Most of the elongated single cells pulled apart eventually due to further stretching across the pores, whereas the large cell made membranes persisted and either slowly migrated away, or were pulled out of the pores after further culture for 10 14 days, which suggested the necessity to also observe cell behaviors on non-pore areas.
It is absolute necessity to maintain them.
Alternatively, the reduced frequency of translocation may reflect the necessity for sufficient levels of Rad52 at both substrates to observe wild type frequencies of DSB-stimulated translocation.
Cooperator clustering can be difficult to observe in small systems, hence the necessity of studying larger ones and, in addition, for times as long as possible [17].
This argument, however, has been criticized as failing to observe the distinction between logical and ontological, or factual, necessity.
While wholeheartedly embracing the reality of charisma, of fame as a psychic necessity for the well-being of the not-at-all-famous, I have to observe a worrying sea-change.
It's fun to observe that giving rail passengers better connectivity takes away one of the key claimed benefits of HS2 – the necessity of getting businesspeople to the ends of the line faster.
MPs like to observe and be observed.
To observe it.
Park to observe elephants.
To observe, not join.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com